济宁青山羊子宫内ERα、ERβ和PR的分布及其mRNA表达的增龄性变化

doi:10.11843/j.issn.0366-6964.2013.10.005

Abstract

Abstract:

To detect the location of ERα, ERβ and PR and the expression of ERα, ERβ and PR mRNA in the uterus of Jining Grey goats during postnatal development. In the uterus of different group, the SABC methods were used to study the distribution of ERα, ERβ and PR, and the real time fluorescent quantitative PCR were used to research the relative expression of ERα, ERβ and PR mRNA with GAPDH as an internal gene. ERα，ERβ and PR were malinly localized in the nuclei of the luminal epithelium, glandular epithelium, stromal cells, endothelium and smooth muscle cells of the uterus，and occasionally in the cytoplasm. The trends of the optical density in the immunopositive cells of ERα, ERβ and PR were similar with that of the expression of ERα, ERβ and PR mRNA. But the level of the expression of ERβ was less than that of ERα. From the birth day to the 30th day, the expression of ERα and ERβ remained at a lower level then increased gradually on the 60th day, ERα and ERβ reached its peak from the 90th day to the 120th day, and afterwards maintained at a high level. While the expression of PR was higher on the birth day, than reached its lowest level on the 30th day and significantly increased after puberty, and two peaks were found during the sexual maturity and maturation. ERα, ERβ and PR have an important role in regulating the proliferation and differentiation of the endometrial and the myometrial.

CLC Number:

S827
S813.3

BAI Shu, TONG Hai-ni, SHI Yun-zhi, HUANG Li-bo, HOU Yan-meng,WANG Shu-ying. Distribution of ERα, ERβ and PR and Expression of Its mRNA in the Uterus of Jining Grey Goats Associated with Aging[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(10): 1539-1546.

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Distribution of ERα, ERβ and PR and Expression of Its mRNA in the Uterus of Jining Grey Goats Associated with Aging

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